CV
Download PDF: Resume
Contact: mdaddysman [at] gmail.com
Work Experience
- Nikon Instruments, Feb 2023 - Present
- Field Applications Scientist
- Chicago, IL
- Agilent Technologies, Nov 2017 - Feb 2023
- Field Applications Scientist
- Chicago, IL
- University of Chicago, Jan 2014 - Nov 2017
- Postdoctoral Scholar
- Chicago, IL
- University of North Carolina, Aug 2009 - Dec 2013
- Graduate Teaching & Research Assistant
- Chapel Hill, NC
Education
- Ph.D. in Physical Chemistry, 2013
- B.S. in Chemistry & Biology, Summa cum Laude, 2009
- Alderson Broaddus University, Philippi, WV
- Minor: International Studies
- Research Advisor: Dr. Yi Charlie Chen, Professor of Biology
Research Experience
- University of Chicago, Institute for Biophysical Dynamics
- Biophysics of diabetes investigated using live cell fluorescence microscopy, December 2013 — Present
- Principle Investigator: Dr. Norbert Scherer, Professor of Chemistry
- Collaborators: Dr. Louis Philipson, Professor of Medicine; Dr. Aaron Dinner, Professor of Chemistry
- Research Summary: My research involves understanding the origins of diabetes at a cellular level. Previous research indicates that some cases of diabetes may be cause by abnormal insulin trafficking in pancreatic beta cells. To further understand the origins of the disease, I use live cell confocal microscopy to image the trafficking of insulin granules. I apply tracking software to obtain trajectories of the motion of granules over time. These trajectories are used to model beta cell behavior under different conditions or stresses. Additionally, I am developing new fluorescent microscope technologies to track cellular structures in 3-D. These technologies increase the axial field of view and resolution of the insulin tracking.
- University of North Carolina at Chapel Hill
- Live cell fluorescence microscopy methods for studying nuclear protein dynamics, December 2009 — December 2013
- Principle Investigator: Dr. Chris Fecko, Assistant Professor of Chemistry
- Research Summary: My research focused on using multiphoton fluorescence microscopy techniques to interrogate DNA-protein interactions in live cells. I developed and characterized a method for creating two-photon photolesions on DNA in live cells as localized binding sites for DNA repair enzymes. My research used the specialized polytene cells of the Drosophila melanogaster larva salivary glands. In these cells, I demonstrated the recruitment of the enzyme topoisomerase I tagged with GFP to the site of DNA damage. Furthermore, I developed a live cell fluorescence recovery after photobleaching method and model to quantitatively measure diffusion and binding of proteins. The model takes into account fluorophore photophysics to more accurately determine protein diffusion rates and the nature of diffusion in disordered systems such as cells. I found that the primary source of anomalous diffusive behavior in cell nuclei for GFP is the dense chromatin network. Finally, I investigated how large protein complexes diffuse in live cells and the quantitative behavior of topoisomerase I recruitment to sites of DNA damage.
- Alderson Broaddus University and WV-INBRE
- Ovarian & prostate cancer suppression by VEGF inhibition with flavonoids, May 2007 — August 2007; January 2008 — August 2008
- Principle Investigator: Dr. Yi Charlie Chen, Professor of Biology
- Research Summary: My research studied ovarian and prostate cancer cell lines that were resistant to cisplatin, the cancer drug commonly used to treat these cancers. Our group focused on flavonoids, a class of antioxidant phytochemicals, thought to be one of the active chemical agents responsible for the link between diets high in fruit and vegetable consumption and the reduced risk of cancer. We looked at two possible methods for the use of flavonoids. First, we measured the flavonoids’ suppression of cancer cell proliferation and angiogenesis, the growth of new blood vessels to support the developing tumor. Secondly, we looked at the interaction of flavonoids with cisplatin to enhance the effectiveness of cisplatin in inducing apoptosis in cell lines mostly resistant to cisplatin. We found that a particular flavonoid, kaempferol, limited angiogenesis in ovarian cancer cells and enhanced cisplatin’s effectiveness in producing apoptosis.
Technical Skills
- Maintains a Yokogawa W1 spinning disk confocal microscope
- Trains group members on the equipment
- Manages web based schedule calendar
- Maintains performance and alignment of the microscope
- Microscopy techniques: Laser scanning (two-photon & confocal), spinning disk confocal, multifocal, interferometric super-resolution, live-cell imaging
- Microscopy software: ImageJ, Micromanager, Slidebook, Huygens
- Programming languages: Matlab, R, Labview, C, C++
- Statistical analysis using R & SPSS
- Biomedical techniques: Immunostaining, PCR, Western blot, cell transfection, tissue culture
- Microsoft Office: Word, Excel, Powerpoint
Publications
Gdor I, Wang X, Daddysman MK, Yifat Y, Wilton R, Hereld M, Noirot-Gros MF and Scherer NF (2018) "Particle tracking by repetitive phase-shift interferometric super resolution microscopy", Opt Lett, 43:2819-2822.
Banerjee S, Lo K, Daddysman MK, Selewa A, Kuntz T, Dinner AR and Scherer NF (2017) "Biphasic growth dynamics control cell division in Caulobacter crescentus", Nat Microbiol, 2:17116.
Huynh T, Daddysman MK, Bao Y, Selewa A, Kuznetsov A, Philipson LH and Scherer NF (2017) "Correlative imaging across microscopy platforms using the fast and accurate relocation of microscopic experimental regions (FARMER) method", Rev Sci Instrum, 88(5):053702.
Daddysman MK, Tycon MA and Fecko CJ (2014) "Photoinduced damage resulting from fluorescence imaging of live cells", Methods Mol Bio, 1148, 1-17.
Tycon MA, Daddysman MK and Fecko CJ (2014) "RNA polymerase II subunits exhibit a broad distribution of macromolecular assembly states in the interchromatin space of cell nuclei", J Phys Chem B, 118:2, 423-433.
Daddysman MK and Fecko CJ (2013) "Revisiting point FRAP to quantitatively characterize anomalous diffusion in live cells", J Phys Chem B, 117:5, 1241-1251.
Daddysman MK and Fecko CJ (2011) "DNA multiphoton absorption generates localized damage for studying repair dynamics in live cells", Biophys J, 101:9, 2294-2303.
Luo H, Daddysman MK, Rankin GO, Jiang BH and Chen YC (2010) "Kaempferol enhances cisplatin's effect on ovarian cancer cells through promoting apoptosis caused by down regulation of cMyc", Cancer Cell Int, 10:16.
Luo H, Rankin GO, Liu L, Daddysman MK, Jiang BH and Chen YC (2009) "Kaempferol inhibits angiogenesis and VEGF expression through both HIF dependent and independent pathways in human ovarian cancer cells", Nutr Cancer, 61:4, 554 – 563.
Talks
August 25, 2016
Talk at 252nd National Meeting of the American Chemical Society, Philadelphia, PA
June 12, 2016
Poster at 76th Scientific Sessions American Diabetes Association, New Orleans, LA
April 01, 2013
Poster at 245th National Meeting of the American Chemical Society, New Orleans, LA
February 03, 2013
Poster at 57th Annual National Meeting of the Biophysical Society, Philadelphia, PA
November 14, 2012
Poster at 64th Southeastern Regional Meeting of the American Chemical Society, Raleigh, NC
August 09, 2012
Talk at UNC Lasers and Microscopy Users Group, Chapel Hill, NC
Teaching
Honors, Awards, and Activites
- University of Chicago
- University of North Carolina
- UNC Chemistry Albert R. Ledoux Teaching Award, 2012
- Alderson Broaddus University
- Outstanding Senior Award, 2009
- Outstanding Natural Science Senior, 2009
- Outstanding Senior Male on Campus Resident, 2009
- Member of Silver Key – Academic Honor Society, 2008
- Outstanding Junior Chemistry Major, 2008
- Appalachian College Association STEM/NSF Scholarship, 2008 — 2009
- Honor Program, 2005 – 2009
- Dean’s List – 8 semesters, 2005 – 2009
- International Study at Alderson Broaddus
- Belize: Rain Forest and Coral Reef Ecology — 3 weeks, 2009
- A Semester in Europe: European History and Culture
- Salzburg, Austria with travel to London, Paris, Rome, Florence, Prague, Amsterdam, Athens, and more.
- Fall 2007, 3 months
- Alderson Broaddus Student Government
- Student Government President, 2008 — 2009
- Student Government Senator, 2006 — 2008
- Valedictorian Philip Barbour High School Class of 2005 — Philippi, WV
- Philip Barbour High School Band — Volunteer Instructor, 2005 – 2017
